Part:BBa_K2354006:Design

Seq Modification :

-Switch codon to avoid EcoRI, XbaI, SpeI, PstI, SacI, NheI, BglI, BamHI, NotI

-Add His tag x6 for protein purification purpose.

-Switch codon to add SacI ( Split the gene to be less than 1KB ).

-Add BamHI at 3’ terminal to facilitate protein fusion.

-Add 3bps at biobrick prefix and subfix to promote cutting effectness.